LOCUS       sequence-85145-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mOrange2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..149
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..881
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
      121 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca
      181 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcgcatgg
      241 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 agggctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      361 acatcctgtc ccctcatttc acctacggct ccaaggccta cgtgaagcac cccgccgaca
      421 tccccgacta cttcaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      481 acgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      541 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg atgcagaaga
      601 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggt gccctgaagg
      661 gcaagatcaa gatgaggctg aagctgaagg acggcggcca ctacacctcc gaggtcaaga
      721 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
      781 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      841 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc
      901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      961 ccccctgaac ctgaaacata aaatgaatgc aatt
//
