LOCUS       sequence-85146-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..149
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..881
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
      121 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca
      181 agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg
      241 agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg
      301 acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg
      361 acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac cccgacgaca
      421 tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct
      481 tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
      541 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
      601 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
      661 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
      721 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
      781 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
      841 cccgcaactc caccgacggc atggacgagc tgtacaagta agcggccgcg actctagatc
      901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaaacc tcccacacct
      961 ccccctgaac ctgaaacata aaatgaatgc aatt
//
