LOCUS       sequence-85147-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    87..167
                     /label=MCS
                     /note="multiple cloning site"
     CDS             175..852
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
      121 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagt
      181 gcgattaagc cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac
      241 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
      301 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
      361 tacggcaaca gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg
      421 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
      481 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg attttatggt
      541 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
      601 actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg atattcatat ggctttgttg
      661 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
      721 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
      781 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
      841 aatgccagac gatccggatc caagctgtag cggccgcgac tctagatcat aatcagccat
      901 accacatttg tagaggtttt acttgcttta aaaaaacctc ccacacctcc cccctgaacc
      961 tgaaacataa aatgaatgca attgttgttg ttaa
//
