LOCUS       sequence-85148-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 548, Emission:
            562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mOrange-pBAD
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             167..877
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     580..599
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagcatgg tgagcaaggg
      181 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
      241 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      301 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      361 cctgtcccct cagttcacct acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      421 cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      481 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      541 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      601 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      661 gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg tcaagaccac
      721 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcggca tcaagttgga
      781 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      841 ccactccacc ggcggcatgg acgagctgta caagtaagaa ttcgaagctt ggctgttttg
      901 gcggatgaga gaagattttc agc
//
