LOCUS       sequence-85149-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 472, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MiCy-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..73
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     24..44
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    96..176
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      178..187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..882
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
ORIGIN
        1 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
       61 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
      121 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      181 accatggtgt cttattcaaa gcaaggcatc gcacaagaaa tgcggacgaa ataccgtatg
      241 gaaggcagtg tcaatggcca tgagttcacg atcgaaggtg taggaactgg aaacccttac
      301 gaagggaaac agatgtccga attagtgatc atcaagccta agggaaaacc ccttccattc
      361 tcctttgaca tactgtcaac agcctttcaa tatggaaaca gatgcttcac aaagtaccct
      421 gccgacatgc ctgactattt caagcaagca ttcccagatg gaatgtcata tgaaaggtca
      481 tttctatttg aggatggagg agttgctaca gccagctgga gcattcgtct cgaaggaaat
      541 tgcttcatcc acaattccat ctatcatggc gtaaactttc ccgctgatgg acccgtaatg
      601 aagaagcaga caattggctg ggataagtcc ttcgaaaaaa tgagtgtggc taaagaggtg
      661 ctaagaggtg atgtgactca gtttcttctg ctcgaaggag gtggttacca gagatgccgg
      721 tttcactcca cttacaaaac ggagaagcca gtcgcaatgc ccccgagtca tgtcgtagaa
      781 catcaaattg tgaggaccga ccttggccaa actgcaaaag gcttcaaggt caagctggaa
      841 gaacatgctg aggctcatgt taaccctttg aaggttaaat aagcggccgc gactctagat
      901 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      961 ccccctgaac ctgaaacata aaatgaatgc aatt
//
