LOCUS       sequence-85150-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 503, Emission: 514, 
            reversibly switches on/off with UV. (Originally reported as 
            Excitation: 503 / 569, Emission: 514 / 581).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos-M-N1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    15..95
                     /label=MCS
                     /note="multiple cloning site"
     CDS             103..783
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tgaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
       61 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatgagtgc gattaagcca
      121 gacatgaaga tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
      181 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
      241 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattcatgta cggcaacagg
      301 gtattcgcca aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg
      361 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac
      421 ataacaatgg aaggggacac tttctataat aaagttcgat tttatggtac caactttccc
      481 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
      541 tatgtgcgtg atggagtgct gacgggtgat attcatatgg ctttgttgct tgaaggaaat
      601 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
      661 ccaggctacc actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac
      721 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
      781 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
      841 tttaaaaaac ctcccacacc tcccccctga acctgaaaca taaaatgaat gcaattgttg
      901 ttgttaactt gtttattgca gct
//
