LOCUS       sequence-85151-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mEos-A69V-N1
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..94
                     /label=MCS
                     /note="multiple cloning site"
     CDS             102..782
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    904..1024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     994..1013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgcgggcc cgggatccac cggtcgccac catgagtgcg attaagccag
      121 acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
      181 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg
      241 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
      301 tattcgtcga atatccagac cacatacaag actattttaa gcagtcgttt cctaaggggt
      361 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca
      421 taacaatgga aggggacact ttctataata aagttcgatt tcacggtaca aactttcccg
      481 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
      541 atgtgcgtga tggagtgctg acgggtgata ttcacatggc tttgttgctt gaaggaaatg
      601 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
      661 caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca
      721 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat
      781 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
      841 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      901 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaatttca
      961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1021 ctta
//
