LOCUS       sequence-85153-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKikGR-N1
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..94
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..803
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     polyA_signal    925..1003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(961..980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagt gtgattacat
      121 cagaaatgaa gatcgagctg cgtatggaag gctctgtaaa cgggcacaag ttcgtgatta
      181 cagggaaagg aagtggcaga ccttacgagg gaacacagac tgtggacctg acagtcatag
      241 agggcggacc tcttcctttt gctttcgata tcctgacaac agcattccat tacggcaacc
      301 gggtatttgt cgaataccca gaagaaatag tagactactt caagcagttg tttcctgagg
      361 gttattcttg ggaacgaagc atgagttacg aagacggggg aatttgcctc gccacaaaca
      421 atataacgat gaagaaagac ggcagcaaca cttttgtcaa tgaaattcga tttgatggta
      481 caaactttcc tgccaatggt ccagttatgc agaggaagac cgtcaaatgg gagccatcca
      541 ctgagaaaat gtatgtgcgt gatggagtgc tgaagggtga tgttgaaatg gctctgttgc
      601 ttgaaggagg tggccattac cgatgtgact tcagaactac ttacaaagca aagaaggttg
      661 tccagttgcc agactatcac tacgtggatc atcaaatgga gataacaagc catgacaagg
      721 attacaacaa ggttaaggcg tatgaacatg ctaaggctta ttccgggacc tacaggggcg
      781 ccaagtatga atttgaagcc taagcggccg cgactctaga tcataatcag ccataccaca
      841 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      901 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaataaa
      961 gcaatagcat cacaaatttc acaaataagc attttttcac tgcattcta
//
