LOCUS       sequence-85158-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-C1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..110
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     61..81
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..859
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    860..925
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1049..1132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1086..1105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
       61 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      121 accgtcagat ccgctagcgg tggccaccat ggtgtctaag ggcgaagagc tgattaagga
      181 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
      241 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      301 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
      361 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
      421 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      481 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
      541 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
      601 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
      661 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
      721 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
      781 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
      841 actggggcac aagcttaatt ccggactcag atctcgagct caagcttcga attctgcagt
      901 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
      961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttttcact gcattc
//
