LOCUS       sequence-85160-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 558, Emission:
            605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-pBAD
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             89..111
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             131..148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             152..184
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             224..904
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     primer_bind     complement(953..970)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ttatccataa gattagcgga tactactgac gcttttatcg caactctcta ctgtttctcc
       61 atacccgttt ttttgggcta gaaataattt tgtttaactt taagaaggag atatacatat
      121 gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      181 gggtcgggat ctgtacgaga acctgtactt gcagggctcg agcatgaaca gcctgatcaa
      241 agaaaacatg cggatgaagg tggtgctgga aggcagcgtg aacggccacc agttcaagtg
      301 caccggcgag ggcgagggca acccctacat gggcacccag accatgcgga tcaaagtgat
      361 cgagggcgga cctctgccct tcgccttcga catcctggcc acatccttca tgtacggcag
      421 ccggaccttc atcaagtacc ccaagggcat ccccgatttc ttcaagcaga gcttccccga
      481 gggcttcacc tgggagagag tgaccagata cgaggacggc ggcgtgatca ccgtgatgca
      541 ggacaccagc ctggaagatg gctgcctggt gtaccatgcc caggtcaggg gcgtgaattt
      601 tcccagcaac ggcgccgtga tgcagaagaa aaccaagggc tgggagccca acaccgagat
      661 gatgtacccc gctgacggcg gactgagagg ctacacccac atggccctga aggtggacgg
      721 cggagggcac ctgagctgca gcttcgtgac cacctaccga tccaagaaaa ccgtgggcaa
      781 catcaagatg cccggcatcc acgccgtgga ccaccggctg gaaaggctgg aagagtccga
      841 caacgagatg ttcgtggtgc agcgggagca cgccgtggcc aagttcgccg gcctgggcgg
      901 agggtaagaa ttcgaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
      961 agattaaatc agaacgcaga agcgtctata atca
//
