LOCUS       sequence-85161-e        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias 
            DA, Tsien RY
  TITLE     A monomeric red fluorescent protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.
  PUBMED    12060735
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-06-11"; volume: "99(12)"; pages: 
            "7877-82"
COMMENT     Sequence Label: mRFP1-C1
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..785
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             111..785
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(241..259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     509..528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     703..726
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    786..851
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    975..1054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1012..1031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1064..1083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttcaaatgtg tacactcgcc catgacgcaa tggcggtagc gtgtcgtgga gtctatataa
       61 gcagagctgg ttagtgaccg tcagatccgc tagcgctacc ggtcgccacc atggcctcct
      121 ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
      181 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      241 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc
      301 agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      361 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      421 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      481 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      541 aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga
      601 ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca
      661 agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc
      721 acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg
      781 gcgcctccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      841 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
      901 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      961 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1021 tcacaaattt cacaataagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1081 atcatgtatc ttaacgcgta aattgtagcg t
//
