LOCUS       sequence-85162-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mWasabi-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..83
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     34..54
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    106..186
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             194..904
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
       61 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga
      121 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
      181 accggtcgcc accatggtga gcaagggcga ggagaccaca atgggcgtaa tcaagcccga
      241 catgaagatc aagctgaaga tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg
      301 cgagggcgag ggcaagccct acgacggcac caacaccatc aacctggagg tgaaggaggg
      361 agcccccctg cccttctcct acgacattct gaccaccgcg ttcagttacg gcaacagggc
      421 cttcaccaag taccccgacg acatccccaa ctacttcaag cagtccttcc ccgagggcta
      481 ctcttgggag cgcaccatga ccttcgagga caagggcatc gtgaaggtga agtccgacat
      541 ctccatggag gaggactcct tcatctacga gatacacctc aagggcgaga acttcccccc
      601 caacggcccc gtgatgcaga aggagaccac cggctgggac gcctccaccg agaggatgta
      661 cgtgcgcgac ggcgtgctga agggcgacgt caagatgaag ctgctgctgg agggcggcgg
      721 ccaccaccgc gttgacttca agaccatcta cagggccaag aaggcggtga agctgcccga
      781 ctatcacttt gtggaccacc gcatcgagat cctgaaccac gacaaggact acaacaaggt
      841 gaccgtttac gagatcgccg tggcccgcaa ctccaccgac ggcatggacg agctgtacaa
      901 gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      961 ctttaaaaac ctcccacacc tccccctgaa cctg
//
