LOCUS       sequence-85163-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 514, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Topaz-N1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..83
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     34..54
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    106..186
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(194..196,197..199,200..913)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(239..260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(500..519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     847..868
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
       61 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga
      121 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
      181 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      361 gccctggccc accctcgtga ccaccttcgg ctacggcgtg cagtgcttcg cccgctaccc
      421 cgaccacatg cgccagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      781 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
      841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      901 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
      961 ttttacttgc tttaaaaacc tcccacacct ccccctgaac ctgaacataa atgaatgcat
     1021 tgttgttgta acttgttatt gcagcttata tggttacaat aagca
//
