LOCUS       sequence-85165-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..54
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     5..25
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..809
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    810..875
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
       61 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc
      121 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat
      181 tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca
      241 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt
      301 atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct
      361 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg
      421 tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg
      481 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata
      541 cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag
      601 ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg
      661 tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg
      721 aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc
      781 ttggtggtgg gatggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga
      841 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgt
//
