LOCUS       sequence-85166-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKO2-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    15..95
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..756
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ttgacgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
       61 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag tgtgattaaa
      121 ccagagatga agatgaggta ctacatggac ggctccgtca atgggcatga gttcacaatt
      181 gaaggtgaag gcacaggcag accttacgag ggacatcaag agatgacact acgcgtcaca
      241 atggccgagg gcgggccaat gcctttcgcg tttgacttag tgtcacacgt gttctgttac
      301 ggccacagag tatttactaa atatccagaa gagataccag actatttcaa acaagcattt
      361 cctgaaggcc tgtcatggga aaggtcgttg gagttcgaag atggtgggtc cgcttcagtc
      421 agtgcgcata taagccttag aggaaacacc ttctaccaca aatccaaatt tactggggtt
      481 aactttcctg ccgatggtcc tatcatgcaa aaccaaagtg ttgattggga gccatcaacc
      541 gagaaaatta ctgccagcga cggagttctg aagggtgatg ttacgatgta cctaaaactt
      601 gaaggaggcg gcaatcacaa atgccaaatg aagactactt acaaggcggc aaaagagatt
      661 cttgaaatgc caggagacca ttacatcggc catcgcctcg tcaggaaaac cgaaggcaac
      721 attactgagc aggtagaaga tgcagtagct catagctccg gatccaagct gtagcggccg
      781 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      841 ctcccacacc tc
//
