LOCUS       sequence-85167-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    21..101
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..840
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtctaag
      121 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
      181 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      241 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      301 agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc cgatttcttt
      361 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      421 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      481 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      541 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgacatg
      601 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
      661 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa
      721 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
      781 tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag
      841 tccggatcca agctgtagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      901 gaggttttac ttgctttaaa aacctcccac acctccccct gaacctgaaa cataaatgaa
      961 tgcaattgtt gttgttaact tgtttattgc agct
//
