LOCUS       sequence-85174-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 487, Emission:
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Emerald-pBAD
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             148..170
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             211..243
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(283..285,286..288,289..1002)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(328..349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(589..608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     936..957
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1048..1065)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ccgccaaaaa gtccacattg attattgcac gcgtcacact ttgctatgcc atagcatttt
       61 tatccataag attagcgata ctacctgacg ctttttatcg caactctcta ctgtttctcc
      121 atacccgttt tttgggctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      181 cggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      241 ggtcgggatc tgtacgagaa cctgtacttc cagggctcga gcatggtgag caagggcgag
      301 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      361 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      421 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc
      481 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      541 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      601 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      661 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      721 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
      781 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      841 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      901 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      961 gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattcga agcttggctg
     1021 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatc
//
