LOCUS       sequence-85179-e        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 684, Emission: 708.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien
            RY
  TITLE     Mammalian expression of infrared fluorescent proteins engineered 
            from a bacterial phytochrome.
  JOURNAL   Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683.
  PUBMED    19423828
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-05-8- 8"; volume: "324"; issue: 
            "5928"; pages: "804-7"
COMMENT     Sequence Label: IFP1.4-C1
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..1010
                     /codon_start=1
                     /product="bacteriophytochrome-based infrared fluorescent 
                     protein (Shu et al., 2009)"
                     /label=IFP1.1
                     /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEA"
     misc_feature    1011..1076
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gccctttcta gttgacgtca gatccgctag cgctaccggt cgccaccatg gctcgggacc
       61 ctctgccatt ctttccacct ctgtacctgg gcggccctga gattacaacc gagaactgcg
      121 agagagagcc tatccacatt cctgggtcca tccagccaca cggggctctg ctcacagctg
      181 acggccactc cggcgaggtg ctccaagtgt ccctgaatgc cgctaccttc ctgggccagg
      241 agcctactgt gctgcggggg cagaccctgg ctgccctgct ccccgagcag tggccagccc
      301 tgcaggcagc cctgccccca ggatgtccag atgccctcca atacagggcc accctcgact
      361 ggccagctgc tgggcacctc agcctgactg tgcatcgggt ggctgaactc ctgatcctgg
      421 agttcgaacc taccgaggcc tgggacagca ttggccctca cgccctgagg aacgccatgt
      481 ttgctctgga aagcgctcca aacctgcggg ctctggccga agtcgcaaca caaactgtga
      541 gagaactgac tggcttcgat cgggtgatgc tgtacaaatt tgcccctgac gccactggag
      601 agatgattgc tgaggccaga cgggagggca tgcaggcttt tctgggccac aggtttcccg
      661 catccaccac ccctgcacaa gctagggccc tctacacaag acacctgctc cggctgaccg
      721 cagacaccag ggctgcagca gtgcccctcg accccgtgct gaatccccag acaaatgctc
      781 ctacacctct gggcggagct gtcctcagag ctacatcccc aatgcacatg cagtacctga
      841 ggaatatggg agtgggctcc tccctgagcg tcagcgtcgt ggtcggcggc cagctgtggg
      901 gactgattgt ctgccaccat cagacaccct acgtgctgcc acccgatctg cggaccaccc
      961 tggaggagct ggggagaaag ctgtccgggc aggtgcagag gaaagaagcc tccggactca
     1021 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     1081 ggatctagat actgatcata tcagccatac cacatttgta gaaggtttta ctgcttaaaa
     1141 cctccccaca cctcccccct gactgaaaca taaatgatgc attgtttgtt gttacttgtt
     1201 attgcagctt atatgtacaa tagcatgcat acaatcaaat taagcatttt tcactgcatt
     1261 ctaatggtgt gtgtccaaac ctc
//
