LOCUS       sequence-85196-e        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 460, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, 
            Verkhusha VV
  TITLE     Monomeric red fluorescent proteins with a large Stokes shift.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74. Epub 2010 Mar
            8.
  PUBMED    20212155
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-03-23"; volume: "107(12)"; pages: 
            "5369-74. Epub 2010 Mar 8"
COMMENT     Sequence Label: LSSmKate2-N1
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..104
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    936..1017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(973..992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1027..1046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gccatccttg tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catgagcgag
      121 ctgattaagg agaacatgca catgaagctg tacatggaag gcaccgtgaa caaccaccac
      181 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      241 aaggtggtcg agggcggccc tctacccttc gccttcgaca tcttggctac cagcttcatg
      301 tacggcagct acaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
      361 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      421 gctacccagg acaccagcct ccaggacggt tgcctcatct acaacgtcaa gatcagaggg
      481 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccggc
      541 accgagatgc tgtaccccgc tgacggcggc ctggaaggca ggtctgacga cgccctgaag
      601 ctcgtgggcg ggggccacct gatctgcaac ttgaagagca catacagatc caagaaaccc
      661 gctaagaatc tcaaggtgcc cggcgtctac tatgtggacc gaagactgga aagaatcaag
      721 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
      781 ctccctagca aactggggca caagcttaat taaagcggcc gcgactctag atcataatca
      841 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      901 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1021 ctagttgtgg tttgtccaaa ctcatcatgt atctagcgta aatgtagcgt atatttgtaa
     1081 atcgcgtaaa ttttgtaatc agctcatttt tacatagcga atcgcaaatc ccttataatc
     1141 aaagaataag acgaaatagc tgatgttgtt cagttgaaca gagtcctata gactgatcca
     1201 cgtcgaggca acggctatca gcaatgccca tactgaacta ccctatccat gcttttggga
     1261 tcaaggtcct tagac
//
