LOCUS       sequence-85198-e        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A
  TITLE     A green-emitting fluorescent protein from Galaxeidae coral and its 
            monomeric version for use in fluorescent labeling.
  JOURNAL   J Biol Chem. 2003 Sep 5. 278(36):34167-71.
  PUBMED    12819206
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 5. 278(36):34167-71."
COMMENT     Sequence Label: mAzami Green-C1 (mAG)
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        165..216
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     167..187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      255..264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             261..938
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             261..938
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     misc_feature    939..1004
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tacgtatagt catcgctata ccatgtgatg cggtttggca gtacatcaat ggcgtgatag
       61 cggttgactc acgggatttc aagtctcacc catgacgtca atgggagttg tttggcacca
      121 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccatgacgca aatgggcggt
      181 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
      241 tagcgctacc ggtcgccacc atggtgagcg tgatcaagcc cgagatgaag atcaagctgt
      301 gcatgagggg caccgtgaac ggccacaact tcgtgatcga gggcgagggc aagggcaacc
      361 cctacgaggg cacccagatc ctggacctga acgtgaccga gggcgccccc ctgcccttcg
      421 cctacgacat cctgaccacc gtgttccagt acggcaacag ggccttcacc aagtaccccg
      481 ccgacatcca ggactacttc aagcagacct tccccgaggg ctaccactgg gagaggagca
      541 tgacctacga ggaccagggc atctgcaccg ccaccagcaa catcagcatg aggggcgact
      601 gcttcttcta cgacatcagg ttcgacggca ccaacttccc ccccaacggc cccgtgatgc
      661 agaagaagac cctgaagtgg gagcccagca ccgagaagat gtacgtggag gacggcgtgc
      721 tgaagggcga cgtgaacatg aggctgctgc tggagggcgg cggccactac aggtgcgact
      781 tcaagaccac ctacaaggcc aagaaggagg tgaggctgcc cgacgcccac aagatcgacc
      841 acaggatcga gatcctgaag cacgacaagg actacaacaa ggtgaagctg tacgagaacg
      901 ccgtggccag gtactccatg ctgcccagcc aggccaagtc cggactcaga tctcgagctc
      961 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac
     1021 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa acctcccaca
     1081 cctccccctg aacctgaaac ataaatgaat gcaattgttg ttgttaactt gtttattgca
     1141 gcttataatg gttacaataa agcatagcat cacaatttca caataaagca ttttttcact
     1201 gcatctagtg tgttgtcaac tcatcatgt
//
