LOCUS       sequence-85199-e        1177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A
  TITLE     A green-emitting fluorescent protein from Galaxeidae coral and its 
            monomeric version for use in fluorescent labeling.
  JOURNAL   J Biol Chem. 2003 Sep 5. 278(36):34167-71.
  PUBMED    12819206
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 5. 278(36):34167-71."
COMMENT     Sequence Label: mAzami Green-N1 (mAG)
FEATURES             Location/Qualifiers
     source          1..1177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        120..171
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     122..142
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    194..274
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             282..962
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             282..962
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 gcagtacatc atggcgtgat agcgttgact cacgggattt cagtctccac ccatgacgtc
       61 aatggagttg tttgcacaaa tcaacggact tccaaaatgt cgtaacaact cgccccattg
      121 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      181 aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
      241 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc gtgatcaagc
      301 ccgagatgaa gatcaagctg tgcatgaggg gcaccgtgaa cggccacaac ttcgtgatcg
      361 agggcgaggg caagggcaac ccctacgagg gcacccagat cctggacctg aacgtgaccg
      421 agggcgcccc cctgcccttc gcctacgaca tcctgaccac cgtgttccag tacggcaaca
      481 gggccttcac caagtacccc gccgacatcc aggactactt caagcagacc ttccccgagg
      541 gctaccactg ggagaggagc atgacctacg aggaccaggg catctgcacc gccaccagca
      601 acatcagcat gaggggcgac tgcttcttct acgacatcag gttcgacggc accaacttcc
      661 cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaaga
      721 tgtacgtgga ggacggcgtg ctgaagggcg acgtgaacat gaggctgctg ctggagggcg
      781 gcggccacta caggtgcgac ttcaagacca cctacaaggc caagaaggag gtgaggctgc
      841 ccgacgccca caagatcgac cacaggatcg agatcctgaa gcacgacaag gactacaaca
      901 aggtgaagct gtacgagaac gccgtggcca ggtactccat gctgcccagc caggccaagt
      961 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     1021 ttaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1081 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaatttcac
     1141 aataagcatt ttttcactgc attctagttg tggtttg
//
