LOCUS       sequence-85205-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..95
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..180
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             202..882
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 caacgggact ttccaaaaat gtcgtaacaa ctccgccccc attgacgcaa atgggcggta
       61 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      121 agcgccacca tgggcgtggc cgacttgatc aagaagttcg agtccatctc caaggaggag
      181 ggggatccac cggtcgccac catgagtgcg attaagccag acatgaagat caaactccgt
      241 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
      301 tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
      361 tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccaa atatccagac
      421 aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
      481 actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact
      541 ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc agttatgcag
      601 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
      661 acgggtgata ttcatatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
      721 agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac
      781 cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
      841 gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat
      901 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaaac ctcccacacc
      961 tcccccctga acctgaaaca taaaatgaat gcaa
//
