LOCUS       sequence-85206-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4a-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..66
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     17..37
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..791
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     misc_feature    792..857
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
       61 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagtg
      121 cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac gggcaccact
      181 ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc atggatcttg
      241 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt
      301 acggcaacag ggtattcgtt aaatatccag acaacataca agactatttt aagcagtcgt
      361 ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgctatg
      421 ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta
      481 ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca
      541 ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattcatatg gctttgttgc
      601 ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg
      661 gtgtcaagtt accaggctac cactttgtgg accacgccat tgagatttta agccatgaca
      721 aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca
      781 atgccagacg atccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta
      841 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
      901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      961 tgaatgcaat tgttgttgtt aacttgttta ttgc
//
