LOCUS       sequence-85207-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4a-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..65
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    117..197
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      199..208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             205..888
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
       61 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      181 gcccgggatc caccggtcgc caccatggtg agtgcgatta agccagacat gaggatcaaa
      241 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      301 aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg acctctgcct
      361 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgttaaatat
      421 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      481 agcttgactt tcgaagacgg gggcatttgc tatgccagaa acgacataac aatggaaggg
      541 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
      601 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      661 gtgctgacgg gtgatattca tatggctttg ttgcttgaag gaaatgccca ttaccgatgt
      721 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
      781 gtggaccacg ccattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      841 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact
      901 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      961 acacctcccc ctgaacctga aacataaaat gaat
//
