LOCUS       sequence-85208-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4b-C1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..754
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     misc_feature    755..820
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
       61 accggtcgcc accatggtga gtgcgattaa gccagacatg aggatcaaac tccgtatgga
      121 aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca agccttatga
      181 gggaaaacag accatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga
      241 tatcctgacc actgcattcc attacggcaa cagggtattc gtgaaatatc cagacaacat
      301 acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt
      361 cgaagacggg ggcatttgca atgccagaaa cgacataaca atggaagggg acactttcta
      421 taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa
      481 gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg
      541 tgatattgag atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac
      601 tacttacaaa gctaaggaga agggtgtcaa gttaccaggc gcccactttg tggaccacgc
      661 cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt
      721 tgctcattct ggattgcctg acaatgccag acgatccgga ctcagatctc gagctcaagc
      781 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
      841 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      901 ccccctgaac ctgaaacata aaa
//
