LOCUS       sequence-85209-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: mEos4b-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..87
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     38..58
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..172
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             194..877
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
       61 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgccac
      121 catgggcgtg gccgacttga tcaagaagtt cgagtccatc tccaaggagg agggggatcc
      181 accggtcgcc accatggtga gtgcgattaa gccagacatg aggatcaaac tccgtatgga
      241 aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca agccttatga
      301 gggaaaacag accatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga
      361 tatcctgacc actgcattcc attacggcaa cagggtattc gtgaaatatc cagacaacat
      421 acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt
      481 cgaagacggg ggcatttgca atgccagaaa cgacataaca atggaagggg acactttcta
      541 taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa
      601 gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg
      661 tgatattgag atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac
      721 tacttacaaa gctaaggaga agggtgtcaa gttaccaggc gcccactttg tggaccacgc
      781 cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt
      841 tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc tagatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgt
//
