LOCUS       sequence-8521-ex       14263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   McKenzie GJ, Craig NL
  TITLE     Fast, easy and efficient: site-specific insertion of transgenes into
            enterobacterial chromosomes using Tn7 without need for selection of 
            the insertion event.
  JOURNAL   BMC Microbiol. 2006 Apr 28;6:39. doi: 10.1186/1471-2180-6-39.
  PUBMED    16646962
REFERENCE   2  (bases 1 to 14263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-04-28"; volume: "6:39. doi"; pages: " 
            10.1186/1471-2180-6-39"
COMMENT     Sequence Label: pGRG37
FEATURES             Location/Qualifiers
     source          1..14263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..130
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(155..160,161..178,179..185)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             239..919
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(305..324)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1239..1544
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1423..1442
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1585..1709)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2116..2994)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3021..3305
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3228..3247
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9996..10013)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10166..10252
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10344..10371
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10390..10481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10482..10550,10551..11342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10700..10719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(11471..11490)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            11513..11622
                     /note="incP origin of transfer"
     primer_bind     complement(11660..11679)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12362..12584
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             12632..13582
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  14015..14180
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atcaaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
       61 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      121 agtcctatgg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      181 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
      241 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      301 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      361 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
      421 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
      481 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
      541 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
      601 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
      661 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
      721 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
      781 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
      841 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct
      901 agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
      961 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     1021 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     1081 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     1141 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     1201 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     1261 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     1321 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc
     1381 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     1441 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     1501 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     1561 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     1621 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     1681 tctcgttcag ctttcttgta caaagtggtt cgatgggtca atagcggccg ccactcgagc
     1741 acctaggagg cgcgccacgg ccgcgtcgac cccacgcccc tctttaatac gacgggcaat
     1801 ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc
     1861 acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag actttattgt
     1921 catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca ggccacgatg
     1981 cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg
     2041 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
     2101 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     2161 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     2221 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     2281 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     2341 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     2401 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     2461 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     2521 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     2581 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     2641 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     2701 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     2761 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     2821 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     2881 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     2941 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     3001 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     3061 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     3121 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     3181 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     3241 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     3301 tccatacccg tttttttggg ctagcgaatt cgagctcggt acccaagtct taaactagac
     3361 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
     3421 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
     3481 ggcgtggcca agggcatggt aaagactata ttccatggct aacagtacaa gaagttcctt
     3541 cttcaggtcg ttcccaccgt atttattctc ataagacggg acgagtccat catttgctat
     3601 ctgacttaga gcttgctgtt tttctcagtc ttgagtggga gagcagcgtg ctagatatac
     3661 gcgagcagtt ccccttatta cctagtgata ccaggcagat tgcaatagat agtggtatta
     3721 agcatcctgt tattcgtggt gtagatcagg ttatgtctac tgatttttta gtggactgca
     3781 aagatggtcc ttttgagcag tttgctattc aagtcaaacc tgcagcagcc ttacaagacg
     3841 agcgtacctt agaaaaacta gaactagagc gtcgctattg gcagcaaaag caaattcctt
     3901 ggttcatttt tactgataaa gaaataaatc ccgtagtaaa agaaaatatt gaatggcttt
     3961 attcagtgaa aacagaagaa gtttctgcgg agcttttagc acaactatcc ccattggccc
     4021 atatcctgca agaaaaagga gatgaaaaca ttatcaatgt ctgtaagcag gttgatattg
     4081 cttatgattt ggagttaggc aaaacattga gtgagatacg agccttaacc gcaaatggtt
     4141 ttattaagtt caatatttat aagtctttca gggcaaataa gtgtgcagat ctctgtatta
     4201 gccaagtagt gaatatggag gagttgcgct atgtggcaaa ttaatgaggt tgtgctattt
     4261 gataatgatc cgtatcgcat tttggctata gaggatggcc aagttgtctg gatgcaaata
     4321 agcgctgata aaggagttcc acaagctagg gctgagttgt tgctaatgca gtatttagat
     4381 gaaggccgct tagttagaac tgatgaccct tatgtacatc ttgatttaga agagccgtct
     4441 gtagattctg tcagcttcca gaagcgcgag gaggattatc gaaaaattct tcctattatt
     4501 aatagtaagg atcgtttcga ccctaaagtc agaagcgaac tcgttgagca tgtggtccaa
     4561 gaacataagg ttactaaggc tacagtttat aagttgttac gccgttactg gcagcgtggt
     4621 caaacgccta atgcattaat tcctgactac aaaaacagcg gtgcaccagg ggaaagacgt
     4681 tcagcgacag gaacagcaaa gattggccga gccagagaat atggtaaggg tgaaggaacc
     4741 aaggtaacgc ccgagattga acgccttttt aggttgacca tagaaaagca cctgttaaat
     4801 caaaaaggta caaagaccac cgttgcctat agacgatttg tggacttgtt tgctcagtat
     4861 tttcctcgca ttccccaaga ggattaccca acactacgtc agtttcgtta tttttatgat
     4921 cgagaatacc ctaaagctca gcgcttaaag tctagagtta aagcaggggt atataaaaaa
     4981 gacgtacgac ccttaagtag tacagccact tctcaggcgt taggccctgg gagtcgttat
     5041 gagattgatg ccacgattgc tgatatttat ttagtggatc atcatgatcg ccaaaaaatc
     5101 ataggaagac caacgcttta cattgtgatt gatgtgttta gtcggatgat cacgggcttt
     5161 tatatcggct ttgaaaatcc gtcttatgtg gtggcgatgc aggcttttgt aaatgcttgc
     5221 tctgacaaaa cggccatttg tgcccagcat gatattgaga ttagtagctc agactggccg
     5281 tgtgtaggtt tgccagatgt gttgctagcg gaccgtggcg aattaatgag tcatcaggtc
     5341 gaagccttag tttctagttt taatgtgcga gtggaaagtg ctccacctag acgtggcgat
     5401 gctaaaggca tagtggaaag cacttttaga acactacaag ccgagtttaa gtcctttgca
     5461 cctggcattg tagagggcag tcggatcaaa agccatggtg aaacagacta taggttagat
     5521 gcatctctgt cggtatttga gttcacacaa attattttgc gtacgatctt attcagaaat
     5581 aaccatctgg tgatggataa atacgatcga gatgctgatt ttcctacaga tttaccgtct
     5641 attcctgtcc agctatggca atggggtatg cagcatcgta caggtagttt aagggctgtg
     5701 gagcaagagc agttgcgagt agcgttactg cctcgccgaa aggtctctat ttcttcattt
     5761 ggcgttaatt tgtggggttt gtattactcg gggtcagaga ttctgcgtga gggttggttg
     5821 cagcggagca ctgatatagc tagacctcaa catttagaag cggcttatga cccagtgctg
     5881 gttgatacga tttatttgtt tccgcaagtt ggcagccgtg tattttggcg ctgtaatctg
     5941 acggaacgta gtcggcagtt taaaggtctc tcattttggg aggtttggga tatacaagca
     6001 caagaaaaac acaataaagc caatgcgaag caggatgagt taactaaacg cagggagctt
     6061 gaggcgttta ttcagcaaac cattcagaaa gcgaataagt taacgcccag tactactgag
     6121 cccaaatcaa cacgcattaa gcagattaaa actaataaaa aagaagccgt gacctcggag
     6181 cgtaaaaaac gtgcggagca tttgaagcca agctcttcag gtgatgaggc taaagttatt
     6241 cctttcaacg cagtggaagc ggatgatcaa gaagattaca gcctacccac atacgtgcct
     6301 gaattatttc aggatccacc agaaaaggat gagtcatgag tgctacccgg attcaagcag
     6361 tttatcgtga tacgggggta gaggcttatc gtgataatcc ttttatcgag gccttaccac
     6421 cattacaaga gtcagtgaat agtgctgcat cactgaaatc ctctttacag cttacttcct
     6481 ctgacttgca aaagtcccgt gttatcagag ctcataccat ttgtcgtatt ccagatgact
     6541 attttcagcc attaggtacg catttgctac taagtgagcg tatttcggtc atgattcgag
     6601 gtggctacgt aggcagaaat cctaaaacag gagatttaca aaagcattta caaaatggtt
     6661 atgagcgtgt tcaaacggga gagttggaga catttcgctt tgaggaggca cgatctacgg
     6721 cacaaagctt attgttaatt ggttgttctg gtagtgggaa gacgacctct cttcatcgta
     6781 ttctagccac gtatcctcag gtgatttacc atcgtgaact caatgtagag caggtggtgt
     6841 atttgaaaat agactgctcg cataatggtt cgctaaaaga aatctgcttg aattttttca
     6901 gagcgttgga tcgagccttg ggctcgaact atgagcgtcg ttatggctta aaacgtcatg
     6961 gtatagaaac catgttggct ttgatgtcgc aaatagccaa tgcacatgct ttagggttgt
     7021 tggttattga tgaaattcag catttaagcc gctctcgttc gggtggatct caagagatgc
     7081 tgaacttttt tgtgacgatg gtgaatatta ttggcgtacc agtgatgttg attggtaccc
     7141 ctaaagcacg agagattttt gaggctgatt tgcggtctgc acgtagaggg gcagggtttg
     7201 gagctatatt ctgggatcct atacaacaaa cgcaacgtgg aaagcccaat caagagtgga
     7261 tcgcttttac ggataatctt tggcaattac agcttttaca acgcaaagat gcgctgttat
     7321 cggatgaggt ccgtgatgtg tggtatgagc taagccaagg agtgatggac attgtagtaa
     7381 aactttttgt actcgctcag ctccgtgcgc tagctttagg caatgagcgt attaccgctg
     7441 gtttattgcg gcaagtgtat caagatgagt taaagcctgt gcaccccatg ctagaggcat
     7501 tacgctcggg tatcccagaa cgcattgctc gttattctga tctagtcgtt cccgagattg
     7561 ataaacggtt aatccaactt cagctagata tcgcagcgat acaagaacaa acaccagaag
     7621 aaaaagccct tcaagagtta gataccgaag atcagcgtca tttatatctg atgctgaaag
     7681 aggattacga ttcaagcctg ttaattccca ctattaaaaa agcgtttagc cagaatccaa
     7741 cgatgacaag acaaaagtta ctgcctcttg ttttgcagtg gttgatggaa ggcgaaacgg
     7801 tagtgtcaga actagaaaag ccctccaaga gtaaaaaggt ttcggctata aaggtagtca
     7861 agcccagcga ctgggatagc ttgcctgata cggatttacg ttatatctat tcacaacgcc
     7921 aacctgaaaa aaccatgcat gaacggttaa aagggaaagg ggtaatagtg gatatggcga
     7981 gcttatttaa acaagcaggt tagccatgag aaactttcct gttccgtact cgaatgagct
     8041 gatttatagc actattgcac gggcaggcgt ttatcaaggg attgttagtc ctaagcagct
     8101 gttggatgag gtgtatggca accgcaaggt ggtcgctacc ttaggtctgc cctcgcattt
     8161 aggtgtgata gcaagacatc tacatcaaac aggacgttac gctgttcagc agcttattta
     8221 tgagcatacc ttattccctt tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc
     8281 tattcggtta atggagtacc aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc
     8341 ttctagagtt aagagcgata accgctttag atactgccct gattgcgttg ctcttcagct
     8401 aaataggtat ggggaagcct tttggcaacg agattggtat ttgcccgctt tgccatattg
     8461 tccaaaacac ggtgctttag tcttctttga tagagctgta gatgatcacc gacatcaatt
     8521 ttgggctttg ggtcatactg agctgctttc agactacccc aaagactccc tatctcaatt
     8581 aacagcacta gctgcttata tagcccctct gttagatgct ccacgagcgc aagagctttc
     8641 cccaagcctt gagcagtgga cgctgtttta tcagcgctta gcgcaggatc tagggctaac
     8701 caaaagcaag cacattcgtc atgacttggt ggcggagaga gtgaggcaga cttttagtga
     8761 tgaggcacta gagaaactgg atttaaagtt ggcagagaac aaggacacgt gttggctgaa
     8821 aagtatattc cgtaagcata gaaaagcctt tagttattta cagcatagta ttgtgtggca
     8881 agccttattg ccaaaactaa cggttataga agcgctacag caggcaagtg ctcttactga
     8941 gcactctata acgacaagac ctgttagcca gtctgtgcaa cctaactctg aagatttatc
     9001 tgttaagcat aaagactggc agcaactagt gcataaatac caaggaatta aggcggcaag
     9061 acagtcttta gagggtgggg tgctatacgc ttggctttac cgacatgaca gggattggct
     9121 agttcactgg aatcaacagc atcaacaaga gcgtctggca cccgccccta gagttgattg
     9181 gaaccaaaga gatcgaattg ctgtacgaca actattaaga atcataaagc gtctagatag
     9241 tagccttgat cacccaagag cgacatcgag ctggctgtta aagcaaactc ctaacggaac
     9301 ctctcttgca aaaaatctac agaaactgcc tttggtagcg ctttgcttaa agcgttactc
     9361 agagagtgtg gaagattatc aaattagacg gattagccaa gcttttatta agcttaaaca
     9421 ggaagatgtt gagcttaggc gctggcgatt attaagaagt gcaacgttat ctaaagagcg
     9481 gataactgag gaagcacaaa gattcttgga aatggtttat ggggaagagt gagtggttag
     9541 gctagctaca tttaatgaca atgtgcaggt tgtacatatt ggtcatttat tccgtaactc
     9601 gggtcataag gagtggcgta tttttgtttg gtttaatcca atgcaagaac ggaaatggac
     9661 tcgatttact catttgcctt tattaagtcg agctaaggtg gttaacagta caacaaagca
     9721 aataaataag gcggatcgtg tgattgagtt tgaagcatcg gatcttcaac gagccaaaat
     9781 aatcgatttt cctaatctct cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag
     9841 ttcatttatt tacgaagctg aaacaccata tagcaagact cgttatcaca tcccacagtt
     9901 agagctagct cggtcattat ttttagggga tcctctagag tcgacctgca ggcatgcaag
     9961 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
    10021 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
    10081 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
    10141 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
    10201 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
    10261 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
    10321 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
    10381 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
    10741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
    11041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11341 aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc
    11401 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11461 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cgccggccag
    11521 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
    11581 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggcggcat caccggcgcc
    11641 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
    11701 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
    11761 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
    11821 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgatcc
    11881 ccgacagtaa gacgggtaag cctgttgatg ataccgctgc cttactgggt gcattagcca
    11941 gtctgaatga cctgtcacgg gataatccga agtggtcaga ctggaaaatc agagggcagg
    12001 aactgctgaa cagcaaaaag tcagatagca ccacatagca gacccgccat aaaacgccct
    12061 gagaagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg aatccataaa
    12121 aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc agcgaactga
    12181 atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag gaatattcag
    12241 cgatttgccc gagcttgcga gggtgctact taagccttta gggttttaag gtctgttttg
    12301 tagaggagca aacagcgttt gcgacatcct tttgtaatac tgcggaactg actaaagtag
    12361 tgagttatac acagggctgg gatctattct ttttatcttt ttttattctt tctttattct
    12421 ataaattata accacttgaa tataaacaaa aaaaacacac aaaggtctag cggaatttac
    12481 agagggtcta gcagaattta caagttttcc agcaaaggtc tagcagaatt tacagatacc
    12541 cacaactcaa aggaaaagga ctagtaatta tcattgacta gcccatctca attggtatag
    12601 tgattaaaat cacctagacc aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa
    12661 tgaactagcg attagtcgct atgacttaac ggagcatgaa accaagctaa ttttatgctg
    12721 tgtggcacta ctcaacccca cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt
    12781 cacttataac caatacgttc agatgatgaa catcagtagg gaaaatgctt atggtgtatt
    12841 agctaaagca accagagagc tgatgacgag aactgtggaa atcaggaatc ctttggttaa
    12901 aggctttgag attttccagt ggacaaacta tgccaagttc tcaagcgaaa aattagaatt
    12961 agtttttagt gaagagatat tgccttatct tttccagtta aaaaaattca taaaatataa
    13021 tctggaacat gttaagtctt ttgaaaacaa atactctatg aggatttatg agtggttatt
    13081 aaaagaacta acacaaaaga aaactcacaa ggcaaatata gagattagcc ttgatgaatt
    13141 taagttcatg ttaatgcttg aaaataacta ccatgagttt aaaaggctta accaatgggt
    13201 tttgaaacca ataagtaaag atttaaacac ttacagcaat atgaaattgg tggttgataa
    13261 gcgaggccgc ccgactgata cgttgatttt ccaagttgaa ctagatagac aaatggatct
    13321 cgtaaccgaa cttgagaaca accagataaa aatgaatggt gacaaaatac caacaaccat
    13381 tacatcagat tcctacctac ataacggact aagaaaaaca ctacacgatg ctttaactgc
    13441 aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga
    13501 tctcaatggt tcgttctcat ggctcacgca aaaacaacga accacactag agaacatact
    13561 ggctaaatac ggaaggatct gaggttctta tggctcttgt atctatcagt gaagcatcaa
    13621 gactaacaaa caaaagtaga acaactgttc accgttacat atcaaaggga aaactgtcca
    13681 tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc agagctttta cgagtttttg
    13741 gtgcatttaa agctgttcac catgaacaga tcgacaatgt aacagatgaa cagcatgtaa
    13801 cacctaatag aacaggtgaa accagtaaaa caaagcaact agaacatgaa attgaacacc
    13861 tgagacaact tgttacagct caacagtcac acatagacag cctgaaacag gcgatgctgc
    13921 ttatcgaatc aaagctgccg acaacacggg agccagtgac gcctcccgtg gggaaaaaat
    13981 catggcaatt ctggaagaaa tagcgctttc agcctgtggg cggacaaaat agttgggaac
    14041 tgggaggggt ggaaatggag tttttaagga ttatttaggg aagagtgaca aaatagatgg
    14101 gaactgggtg tagcgtcgta agctaatacg aaaattaaaa atgacaaaat agtttggaac
    14161 tagatttcac ttatctggtt ggtcgacact agtattaccc tgttatccct agatttaaat
    14221 gatatcggat cctagtaagc cacgttttaa ttaatcagat ccc
//
