LOCUS       sequence-85215-e        1276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEMS-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..122
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             132..812
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    934..1053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(971..990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 acaagctagt gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
       61 gaattcccac catgggtgtc gcagatttga tcaagaaatt cgaaagcatc tcaaaggaag
      121 aacgggatcc aatgagtgcg attaagccag acatgaagat caaactccgt atggaaggca
      181 acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa
      241 aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc
      301 tgaccactgc attcatgtac ggcaacaggg tattcgccaa atatccagac aacatacaag
      361 actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag
      421 acgggggcat ttgcaacgcc agaaacgaca taacaatgga aggggacact ttctataata
      481 aagttcgatt ttatggtacc aactttcccg ccaatggtcc agttatgcag aagaagacgc
      541 tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgatg
      601 tagagatggc tttgttgctt gaaggaaatg cccattaccg atgtgactcc agaactactt
      661 acaaagctaa ggagaagggt gtcaagttac caggcgccca ctttgtggac cactgcattg
      721 agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc
      781 attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc
      841 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      901 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1021 agttgtgttt gtccaaactc atcatgtatc ttacgcgtaa atgtagcgtt atatttttgt
     1081 aaaatcgcgt aaattttttg taaatcagct catttttaca tagcgaaatc ggcaatccta
     1141 taaatcaaag aatagaccga gatagggtgg agggttgttc agttgaacaa gagtcatata
     1201 taaggactgg atccacgtca ggcgaacgct ctatcagggc gatggtcact actgtgaaca
     1261 cactcacacc ctatca
//
