LOCUS       sequence-85216-e        1298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEMS-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        237..438
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     389..409
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             510..560
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             570..1250
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gcattcaata tagagcgtag tgtgttccat atgtaccgcc caataggaac tcccatgagc
       61 gtcatggtgg agatttacgt aactgcccac tggcagtaca ttcaagtgta tcatatgcca
      121 gtacgccccc tattgacgtc atgacggtaa atggcccgcc tggcatatgc ccagtacatg
      181 acttatggga ctttctactg gcagtacatc tacgtattag tcatcgctat accatggtga
      241 tgcgttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      301 tctccacccc attgacgtca atgggagttt gtttggcacc aaaatcaacg ggactttcca
      361 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      421 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag
      481 atctcgagct caagcttcga attcccacca tgggtgtcgc agatttgatc aagaaattcg
      541 aaagcatctc aaaggaagaa cgggatccaa tgagtgcgat taagccagac atgaagatca
      601 aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      661 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      721 cttttgcctt tgatatcctg accactgcat tcatgtacgg caacagggta ttcgccaaat
      781 atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
      841 gaagcttgac tttcgaagac gggggcattt gcaacgccag aaacgacata acaatggaag
      901 gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag
      961 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
     1021 gagtgctgac gggtgatgta gagatggctt tgttgcttga aggaaatgcc cattaccgat
     1081 gtgactccag aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact
     1141 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
     1201 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgataa gcggccgcga
     1261 ctctagatca taatcagcca taccacattt tagaggtt
//
