LOCUS       sequence-85222-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635. Also known as mKate2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: mKate1.31-pBAD
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             174..191
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..227
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             240..260
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             267..965
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     primer_bind     complement(1011..1028)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 catgatattg cacggcgtca cactttgcta tgccatagca ttttatccat aagattagcg
       61 gatactacct gacgctttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      121 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc
      181 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
      241 agaacctgta cttccagggc tcgagcatgg tgagcgagct gattaaggag aacatgcaca
      301 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
      361 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag ggcggccctc
      421 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa accttcatca
      481 accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc ttcacatggg
      541 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
      601 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc
      661 ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg taccccgctg
      721 acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg ggccacctga
      781 tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
      841 gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa gagacctacg
      901 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
      961 gatgagaatt cgaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag
     1021 attaaatc
//
