LOCUS       sequence-85230-e        1288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548 / 634, Emission: 
            565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: PS-mOrange2-N1
FEATURES             Location/Qualifiers
     source          1..1288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..104
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..819
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     719..742
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    944..1025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(981..1000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1035..1054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accatgctag tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc
      121 aagggcgagg agaataatat ggccatcatt aaggagttca tgcgcttcaa ggtgcacatg
      181 gagggcactg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccacccctac
      241 gagggctttc agaccgctaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      301 gacatcctgt cccctctcat cacctacggc tccaaggcct acgtgaagca ccccgccgac
      361 atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      421 tacgaggacg gcggcgtggt gaccgtgacc caggactcct ctctgcagga cggcgagttc
      481 atctacaagg tgaagatgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag
      541 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      601 ggcgagatca ggatgaggct gaagctgaag gatggcggcc actacacctc cgaggtcaag
      661 actacctaca aggccaagaa gtccgtgctg ctgcccggcg cctacatcgt cggcatcaag
      721 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgctccgag
      781 gcccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc gactctagat
      841 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      901 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1021 actgcattct agttgtggtt tgtccaaact catcatgtat ctacgcgtaa attgtagcgt
     1081 tatattttgt aaaatcgcgt aaatttttgt taaatcagct catttttaca tagcgaatcg
     1141 caatccctat aaatcaagga tagacgaaaa ggtgagtgtt gttcagtgga acagagtccc
     1201 tattaggact ggatccacgt caaggcaatc ggctctatca gccaatgcca ctacggtcaa
     1261 ccacttcccg tatatcaagg tttttttg
//
