LOCUS       sequence-85231-e        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548 / 634, Emission: 
            565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: PS-mOrange2-N1
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        318..417
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     368..388
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    440..520
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             528..1235
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     1135..1158
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgggccattg gagcgttcca atatagagcg tagttccata gtaagccaat agacttccat
       61 gagtcatgtg ggaatttccc gtactgccca tgcagtactt caggtattca tatgcagtac
      121 gcccctattg acgtcatgac gtaatgccgc tgcattatgt cagtacatga ctatggactt
      181 tctactgcag tacatctacg tatagtcatc gctatacatg tgatgcgttt gcagtacatc
      241 aatgggcgtg gatagcgttt gactcacggg gatttcaagt ctcaccccat tgacgtcaat
      301 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      361 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      421 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
      481 ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg
      541 gcgaggagaa taatatggcc atcattaagg agttcatgcg cttcaaggtg cacatggagg
      601 gcactgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccac ccctacgagg
      661 gctttcagac cgctaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      721 tcctgtcccc tctcatcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc
      781 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
      841 aggacggcgg cgtggtgacc gtgacccagg actcctctct gcaggacggc gagttcatct
      901 acaaggtgaa gatgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga
      961 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1021 agatcaggat gaggctgaag ctgaaggatg gcggccacta cacctccgag gtcaagacta
     1081 cctacaaggc caagaagtcc gtgctgctgc ccggcgccta catcgtcggc atcaagctgg
     1141 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc tccgaggccc
     1201 gccactccac cggcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata
     1261 atcagccata ccacattgta gagattttcc
//
