LOCUS       sequence-85232-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: tdminiSOG-C1
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..349
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             377..694
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     misc_feature    695..760
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    884..965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(921..940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggagaag agcttcgtga tcaccgaccc
       61 ccgcctgccc gacaacccca tcatcttcgc cagcgacggc ttcctggagc tgaccgagta
      121 cagcagagag gagatcctgg gccgcaacgg ccgcttcctg cagggccccg agaccgacca
      181 ggccaccgtg cagaagatcc gcgacgccat ccgcgaccag cgcgagatca ccgtgcagct
      241 gatcaactac accaagagcg gcaagaagtt ctggaacctg ctgcacctgc agcccatgcg
      301 cgaccagaag ggcgagctgc agtacttcat cggcgtgcag ctggacggct ctggtggcgg
      361 gagtggtggc gggtccatgg agaaaagttt cgtgataact gatccacggc tgccagacaa
      421 tcccatcatc ttcgcatccg atggcttcct ggagctgacc gagtattcca gagaggagat
      481 cctgggccgc aatggccgct ttctgcaggg accagagaca gaccaggcca cagtgcagaa
      541 gattcgcgat gccattagag atcagcgcga gattaccgtg cagctgataa actacacaaa
      601 aagcgggaag aaattctgga acctcctgca cctccagccc atgagggacc agaagggtga
      661 gctccagtat ttcatcggag tgcagctgga tggatccgga ctcagatctc gagctcaagc
      721 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
      781 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      841 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      961 actgcattct agttgtgg
//
