LOCUS       sequence-85234-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: tdminiSOG-N1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..418
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             446..766
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    888..969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(925..944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggagaaga gcttcgtgat
      121 caccgacccc cgcctgcccg acaaccccat catcttcgcc agcgacggct tcctggagct
      181 gaccgagtac agcagagagg agatcctggg ccgcaacggc cgcttcctgc agggccccga
      241 gaccgaccag gccaccgtgc agaagatccg cgacgccatc cgcgaccagc gcgagatcac
      301 cgtgcagctg atcaactaca ccaagagcgg caagaagttc tggaacctgc tgcacctgca
      361 gcccatgcgc gaccagaagg gcgagctgca gtacttcatc ggcgtgcagc tggacggctc
      421 tggtggcggg agtggtggcg ggtccatgga gaaaagtttc gtgataactg atccacggct
      481 gccagacaat cccatcatct tcgcatccga tggcttcctg gagctgaccg agtattccag
      541 agaggagatc ctgggccgca atggccgctt tctgcaggga ccagagacag accaggccac
      601 agtgcagaag attcgcgatg ccattagaga tcagcgcgag attaccgtgc agctgataaa
      661 ctacacaaaa agcgggaaga aattctggaa cctcctgcac ctccagccca tgagggacca
      721 gaagggtgag ctccagtatt tcatcggagt gcagctggat ggataagcgg ccgcgactct
      781 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      841 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      961 tttcactgca ttctagttgt gg
//
