LOCUS       sequence-85235-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: tdminiSOG-N1
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..205
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     156..176
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    228..308
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      310..319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             316..633
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             661..981
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 ccatggtgat gcggtttggc agtacatcaa tgggcgtgga tagcggttga ctcacgggga
       61 ttccaagtct ccaccccatt gacgtcaatg ggagttgttt tggcaccaaa atcaacggga
      121 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      181 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      241 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      301 ccaccggtcg ccaccatgga gaagagcttc gtgatcaccg acccccgcct gcccgacaac
      361 cccatcatct tcgccagcga cggcttcctg gagctgaccg agtacagcag agaggagatc
      421 ctgggccgca acggccgctt cctgcagggc cccgagaccg accaggccac cgtgcagaag
      481 atccgcgacg ccatccgcga ccagcgcgag atcaccgtgc agctgatcaa ctacaccaag
      541 agcggcaaga agttctggaa cctgctgcac ctgcagccca tgcgcgacca gaagggcgag
      601 ctgcagtact tcatcggcgt gcagctggac ggctctggtg gcgggagtgg tggcgggtcc
      661 atggagaaaa gtttcgtgat aactgatcca cggctgccag acaatcccat catcttcgca
      721 tccgatggct tcctggagct gaccgagtat tccagagagg agatcctggg ccgcaatggc
      781 cgctttctgc agggaccaga gacagaccag gccacagtgc agaagattcg cgatgccatt
      841 agagatcagc gcgagattac cgtgcagctg ataaactaca caaaaagcgg gaagaaattc
      901 tggaacctcc tgcacctcca gcccatgagg gaccagaagg gtgagctcca gtatttcatc
      961 ggagtgcagc tggatggata agcggccgcg actctagatc ataatcagcc ataccaca
//
