LOCUS       sequence-85237-e        1294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 553, Emission:
            574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, 
            Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, 
            Chudakov DM
  TITLE     Bright monomeric red fluorescent protein with an extended 
            fluorescence lifetime.
  JOURNAL   Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.
  PUBMED    17572680
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2007-07"; volume: "4(7)"; pages: "555-7. Epub 2007
            Jun 17"
COMMENT     Sequence Label: TurboRFP-pBAD
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     268..287
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             371..393
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             413..430
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             434..466
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             479..499
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             506..1201
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(1247..1264)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cctcagatgc attaccgcca ccagtgccat aacgatcgac ggatctgcta cattttacat
       61 ctcccgccat tcagagagaa ccatgtccaa ttgcatcaga acatggcgtc atggctttaa
      121 ctggcttttt ccgctaacaa cggtaacccg gtattaaaag gcatttgtaa caagcgggac
      181 caaaggccat gacaaaacgg gtaacaaaag tgtctttatc acggcagaaa agtcacattg
      241 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      301 atactactga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc
      361 tagaaataat tttgtttaac tttaagaagg agatatacat atgcggggtt ctcatcatca
      421 tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga
      481 gaacctgtac ttccagggct cgagcatgag cgagctgatc aaggagaaca tgcacatgaa
      541 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
      601 caagccctac gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg gccctctccc
      661 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaagcct tcatcaacca
      721 cacccagggc atccccgact tctttaagca gtccttccct gagggcttca catgggagag
      781 aatcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcttccagaa
      841 cggctgcatc atctacaacg tcaagatcaa cggggtgaac ttcccatcca acggccctgt
      901 gatgcagaag aaaacacgcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg
      961 cggcctgaga ggccacagcc agatggccct gaagctcgtg ggcgggggct acctgcactg
     1021 ctccttcaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggctt
     1081 ccacttcgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     1141 gcagcacgag atggctgtgg ccaagtactg cgacctccct agcaaactgg ggcacagatg
     1201 agaattcgaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     1261 aatcagaacg cagaagcgtc tgataaagca tgag
//
