LOCUS       sequence-85254-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Actin-7
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctatggatg atgatatcgc
      781 cgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg gcgacgatgc
      841 cccccgggcc gtcttcccct ccatcgtggg gcgccccagg caccagggcg tgatggtggg
      901 catgggtcag aaggattcct atgtgggcga cgaggcccag agcaagagag gcatcctcac
      961 cctgaagtac cccatcgagc acggcatcgt caccaactgg gacgacatgg agaaaatctg
     1021 gcaccacacc ttctacaatg agctgcgtgt ggctcccgag gagcaccccg tgctgctgac
     1081 cgaggccccc ctgaacccca aggccaaccg cgagaagatg acccagatca tgtttgagac
     1141 cttcaacacc ccagccatgt acgttgctat ccaggctgtg ctatccctgt acgcctctgg
     1201 ccgtaccact ggcatcgtga tggactccgg tgacggggtc acccacactg tgcccatcta
     1261 cgaggggtat gccctccccc atgccatcct gcgtctggac ctggctggcc gggacctgac
     1321 tgactacctc atgaagatcc tcaccgagcg cggctacagc ttcaccacca cggccgagcg
     1381 ggaaatcgtg cgtgacatta aggagaagct gtgctacgtc gccctggact tcgagcaaga
     1441 gatggccacg gctgcttcca gctcctccct ggagaagagc tacgagctgc ctgacggcca
     1501 ggtcatcacc attggcaatg agcggttccg ctgccctgag gcactcttcc agccttcctt
     1561 cctgggcatg gagtcctgtg gcatccacga aactaccttc aactccatca tgaagtgtga
     1621 cgtggacatc cgcaaagacc tgtacgccaa cacagtgctg tctggcggca ccaccatgta
     1681 ccctggcatt gccgacagga tgcagaagga gatcactgcc ctggcaccca gcacaatgaa
     1741 gatcaagatc attgctcctc ctgagcgcaa gtactccgtg tggatcggcg gctccatcct
     1801 ggcctcgctg tccaccttcc agcagatgtg gatcagcaag caggagtatg acgagtccgg
     1861 cccctccatc gtccaccgca aatgcttcta gggatccacc ggatctagat aactgat
//
