LOCUS       sequence-85255-e        1967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, 
            Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, 
            Waterman CM
  TITLE     Vinculin-actin interaction couples actin retrograde flow to focal 
            adhesions, but is dispensable for focal adhesion growth.
  JOURNAL   J Cell Biol. 2013 Jul 8;202(1):163-77. doi: 10.1083/jcb.201303129.
  PUBMED    23836933
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-07-8- 8"; volume: "202"; 
            issue: "1"; pages: "163-77"
COMMENT     Sequence Label: mApple-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc
      781 ggcggaagcg gaagcgatga tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg
      841 tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc catcgtgggg
      901 cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta tgtgggcgac
      961 gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca cggcatcgtc
     1021 accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga gctgcgtgtg
     1081 gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc
     1141 gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta cgttgctatc
     1201 caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat ggactccggt
     1261 gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca tgccatcctg
     1321 cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct caccgagcgc
     1381 ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg
     1441 tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag ctcctccctg
     1501 gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga gcggttccgc
     1561 tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg catccacgaa
     1621 actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct gtacgccaac
     1681 acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat gcagaaggag
     1741 atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc tgagcgcaag
     1801 tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg
     1861 atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa atgcttctag
     1921 ggatccaccg gatctagata actgatcata atcagccata ccacatt
//
