LOCUS       sequence-85258-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mApple-Annexin(A4)-12
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagaat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
      181 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc
      301 aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc
      361 attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag
      541 aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
      781 atggccatgg caaccaaagg aggtactgtc aaagctgctt caggattcaa tgccatggaa
      841 gatgcccaga ccctgaggaa ggccatgaaa gggctcggca ccgatgaaga cgccattatt
      901 agcgtccttg cctaccgcaa caccgcccag cgccaggaga tcaggacagc ctacaagagc
      961 accatcggca gggacttgat agacgacctg aagtcagaac tgagtggcaa cttcgagcag
     1021 gtgattgtgg ggatgatgac gcccacggtg ctgtatgacg tgcaagagct gcgaagggcc
     1081 atgaagggag ccggcactga tgagggctgc ctaattgaga tcctggcctc ccggacccct
     1141 gaggagatcc ggcgcataag ccaaacctac cagcagcaat atggacggag ccttgaagat
     1201 gacattcgct ctgacacatc gttcatgttc cagcgagtgc tggtgtctct gtcagctggt
     1261 gggagggatg aaggaaatta tctggacgat gctctcgtga gacaggatgc ccaggacctg
     1321 tatgaggctg gagagaagaa atgggggaca gatgaggtga aatttctaac tgttctctgt
     1381 tcccggaacc gaaatcacct gttgcatgtg tttgatgaat acaaaaggat atcacagaag
     1441 gatattgaac agagtattaa atctgaaaca tctggtagct ttgaagatgc tctgctggct
     1501 atagtaaagt gcatgaggaa caaatctgca tattttgctg aaaagctcta taaatcgatg
     1561 aagggcttgg gcaccgatga taacaccctc atcagagtga tggtttctcg agcagaaatt
     1621 gacatgttgg atatccgggc acacttcaag agactctatg gaaagtctct gtactcgttc
     1681 atcaagggtg acacatctgg agactacagg aaagtactgc ttgttctctg tggaggagat
     1741 gattaaggat ccaccggatc tagataactg atcat
//
