LOCUS       sequence-85261-e        2551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2551)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1827..1836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1833..2543
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2246..2265
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atctcgagac cgccatggaa gggaagtggt tgctgtgtat gttactggtg cttggaactg
       61 ctattgttga ggctcatgat ggacatgatg atgatgtgat tgatattgag gatgaccttg
      121 acgatgtcat tgaagaggta gaagactcaa aaccagatac cactgctcct ccttcatctc
      181 ccaaggttac ttacaaagct ccagttccaa caggggaagt atattttgct gattcttttg
      241 acagaggaac tctgtcaggg tggattttat ccaaagccaa gaaagacgat accgatgatg
      301 aaattgccaa atatgatgga aagtgggagg tagaggaaat gaaggagtca aagcttccag
      361 gtgataaagg acttgtgttg atgtctcggg ccaagcatca tgccatctct gctaaactga
      421 acaagccctt cctgtttgac accaagcctc tcattgttca gtatgaggtt aatttccaaa
      481 atggaataga atgtggtggt gcctatgtga aactgctttc taaaacacca gaactcaacc
      541 tggatcagtt ccatgacaag accccttata cgattatgtt tggtccagat aaatgtggag
      601 aggactataa actgcacttc atcttccgac acaaaaaccc caaaacgggt atctatgaag
      661 aaaaacatgc taagaggcca gatgcagatc tgaagaccta ttttactgat aagaaaacac
      721 atctttacac actaatcttg aatccagata atagttttga aatactggtt gaccaatctg
      781 tggtgaatag tggaaatctg ctcaatgaca tgactcctcc tgtaaatcct tcacgtgaaa
      841 ttgaggaccc agaagaccgg aagcccgagg attgggatga aagaccaaaa atcccagatc
      901 cagaagctgt caagccagat gactgggatg aagatgcccc tgctaagatt ccagatgaag
      961 aggccacaaa acccgaaggc tggttagatg atgagcctga gtacgtacct gatccagacg
     1021 cagagaaacc tgaggattgg gatgaagaca tggatggaga atgggaggct cctcagattg
     1081 ccaaccctag atgtgagtca gctcctggat gtggtgtctg gcagcgacct gtgattgaca
     1141 accccaatta taaaggcaaa tggaagcctc ctatgattga caatcccagt taccagggaa
     1201 tctggaaacc caggaaaata ccaaatccag atttctttga agatctggaa cctttcagaa
     1261 tgactccttt tagtgctatt ggtttggagc tgtggtccat gacctctgac attttttttg
     1321 acaactttat catttgtgct gatcgaagaa tagttgatga ttgggccaat gatggatggg
     1381 gcctgaagaa agctgctgat ggggctgctg agccaggcgt tgtggggcag atgaacgagg
     1441 cagctgaaga gcgcccgtgg ctgtgggtag tctatattct aactgtagcc cttcctgtgt
     1501 tcctggttat cctcttctgc tgttctggaa agaaacagac cagtggtatg gagtataaga
     1561 aaactgatgc acctcaaccg gatgtgaagg aagaggaaga agagaaggaa gaggaaaagg
     1621 acaagggaga tgaggaggag gaaggagaag agaaacttga agagaaacag aaaagtgatg
     1681 ctgaagaaga tggtggcact gtcagtcaag aggaggaaga cagaaaacct aaagcagagg
     1741 aggatgaaat tttgaacaga tcaccaagaa acagaaagcc acgaagagag gggggtgcgt
     1801 caggcggcag cggatctgga ccggtcgcca ccatggtgag caagggcgag gagaataaca
     1861 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1921 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     1981 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2041 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
     2101 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
     2161 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
     2221 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2281 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
     2341 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     2401 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     2461 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2521 gcatggacga gctgtacaag taagcggccg c
//
