LOCUS       sequence-85262-e        1822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1822)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1067..1076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1073..1783
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1486..1505
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ccaggatgtg
       61 gtggtccttg atccctctct cttgcctgct ggcactgacc agtgcccatg acaagccttc
      121 ctttcaccca ctgtcggacg acatgattaa ctatatcaac aaacagaata caacatggca
      181 ggctggacgc aacttctaca atgttgacat aagctatctg aagaagctgt gtggaactgt
      241 cctgggtgga cccaagctgc cggaaagggt tgggttcagc gaggacataa atctacctga
      301 atcctttgat gcacgggaac agtggtccaa ttgcccgacc atcgcacaga tcagagacca
      361 ggggtcctgt ggctcttgtt gggcatttgg ggcagtggaa gccatgtctg accgaatctg
      421 cattcacacc aatggccgag tcaatgtgga ggtgtctgct gaggacctgc ttacctgctg
      481 tggtatccag tgtggggatg gctgtaatgg tggctatccc tctggagcat ggaacttctg
      541 gactagaaaa ggcctggttt ctggtggagt atacaattct catataggct gcttacccta
      601 caccatccct ccctgtgaac accatgtcaa tggctcccgt cccccatgca ctggagaagg
      661 agatactccc aagtgcaaca agatgtgtga ggctggctac tccacatcct acaaggaaga
      721 taagcactat gggtacactt cctacagtgt gtctgacagc gagaaggaga tcatggcgga
      781 aatctacaaa aatggcccag tggagggtgc ttttactgtg ttttctgact tcttgactta
      841 caaatcaggc gtatacaagc atgaagccgg tgatgtgatg ggaggccatg ccatccgcat
      901 tctgggctgg ggaatagaga atggagtacc ctactggctg gtagcaaact cctggaacgt
      961 tgactggggt gataatggtt tctttaaaat cctcagagga gagaaccact gtggaattga
     1021 atcagaaatc gtggctggaa tcccacgcac tcaggatcca ccggtcgcca ccatggtgag
     1081 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     1141 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1201 cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1261 ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc tacattaagc acccagccga
     1321 catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa
     1381 cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt
     1441 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1501 gaagaccatg ggctgggagg cctccgagga gcggatgtac cccgaggacg gcgccctgaa
     1561 gagcgagatc aagaagaggc tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa
     1621 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa
     1681 gttggacatc gtgtcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1741 gggccgccac tccaccggcg gcatggacga gctgtacaag taagcggccg cgactctaga
     1801 tcataatcag ccataccaca tt
//
