LOCUS       sequence-85263-e        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctggc agcagtggag gttctggggg
      781 caaatacgta gactcggagg gacatctcta caccgttccc atccgggaac agggcaacat
      841 ctacaagccc aacaacaagg ccatggcaga cgagctgagc gagaagcaag tgtacgacgc
      901 gcacaccaag gagatcgacc tggtcaaccg cgaccctaaa cacctcaacg atgacgtggt
      961 caagattgac tttgaagatg tgattgcaga accagaaggg acacacagtt ttgacggcat
     1021 ttggaaggcc agcttcacca ccttcactgt gacgaaatac tggttttacc gcttgctgtc
     1081 tgccctcttt ggcatcccga tggcactcat ctggggcatt tacttcgcca ttctctcttt
     1141 cctgcacatc tgggcagttg taccatgcat taagagcttc ctgattgaga ttcagtgcat
     1201 cagccgtgtc tattccatct acgtccacac cgtctgtgac ccactctttg aagctgttgg
     1261 gaaaatattc agcaatgtcc gcatcaactt gcagaaagaa atataagaat tctgcagtcg
     1321 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     1381 cattt
//
