LOCUS       sequence-85264-e        1585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1585)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CD151-7
FEATURES             Location/Qualifiers
     source          1..1585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..733
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat
       61 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa
      181 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      241 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt
      301 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt
      361 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac
      421 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga
      481 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct
      541 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt
      601 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
      661 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      721 cgagctgtac aagtccggac tcagatctgg cggtatgggt gagttcaacg agaagaagac
      781 aacatgtggc accgtttgcc tcaagtacct gctgtttacc tacaattgct gcttctggct
      841 ggctggcctg gctgtcatgg cagtgggcat ctggacgctg gccctcaaga gtgactacat
      901 cagcctgctg gcctcaggca cctacctggc cacagcctac atcctggtgg tggcgggcac
      961 tgtcgtcatg gtgactgggg tcttgggctg ctgcgccacc ttcaaggagc gtcggaacct
     1021 gctgcgcctg tacttcatcc tgctcctcat catctttctg ctggagatca tcgctggtat
     1081 cctcgcctac gcctactacc agcagctgaa cacggagctc aaggagaacc tgaaggacac
     1141 catgaccaag cgctaccacc agccgggcca tgaggctgtg accagcgctg tggaccagct
     1201 gcagcaggag ttccactgct gtggcagcaa caactcacag gactggcgag acagtgagtg
     1261 gatccgctca caggaggccg gtggccgtgt ggtcccagac agctgctgca agacggtggt
     1321 ggctctttgt ggacagcgag accatgcctc caacatctac aaggtggagg gcggctgcat
     1381 caccaagttg gagaccttca tccaggagca cctgagggtc attggggctg tggggatcgg
     1441 cattgcctgt gtgcaggtct ttggcatgat cttcacgtgc tgcctgtaca ggagtctcaa
     1501 gctggagcac tactgagaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     1561 ctagataact gatcataatc agcca
//
