LOCUS       sequence-85265-e        2404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2404)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..126
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     77..97
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..878
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     584..603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcaatg ggagtttgtt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
       61 actccgcccc atttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      121 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca
      181 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      241 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      361 acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca
      421 tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact
      481 tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca
      541 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      601 agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga
      661 gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga
      721 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
      781 tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      841 gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga tctggcagca
      901 gtggaggtgg ctgtgaccgg aactgtgggc tcatcgctgg ggctgtcatt ggtgctgtcc
      961 tggctgtgtt tggaggtatt ctaatgccag ttggagacct gcttatccag aagacaatta
     1021 aaaagcaagt tgtcctcgaa gaaggtacaa ttgcttttaa aaattgggtt aaaacaggca
     1081 cagaagttta cagacagttt tggatctttg atgtgcaaaa tccacaggaa gtgatgatga
     1141 acagcagcaa cattcaagtt aagcaaagag gtccttatac gtacagagtt cgttttctag
     1201 ccaaggaaaa tgtaacccag gacgctgagg acaacacagt ctctttcctg cagcccaatg
     1261 gtgccatctt cgaaccttca ctatcagttg gaacagaggc tgacaacttc acagttctca
     1321 atctggctgt ggcagctgca tcccatatct atcaaaatca atttgttcaa atgatcctca
     1381 attcacttat taacaagtca aaatcttcta tgttccaagt cagaactttg agagaactgt
     1441 tatggggcta tagggatcca tttttgagtt tggttccgta ccctgttact accacagttg
     1501 gtctgtttta tccttacaac aatactgcag atggagttta taaagttttc aatggaaaag
     1561 ataacataag taaagttgcc ataatcgaca catataaagg taaaaggaat ctgtcctatt
     1621 gggaaagtca ctgcgacatg attaatggta cagatgcagc ctcatttcca ccttttgttg
     1681 agaaaagcca ggtattgcag ttcttttctt ctgatatttg caggtcaatc tatgctgtat
     1741 ttgaatccga cgttaatctg aaaggaatcc ctgtgtatag atttgttctt ccatccaagg
     1801 cctttgcctc tccagttgaa aacccagaca actattgttt ctgcacagaa aaaattatct
     1861 caaaaaattg tacatcatat ggtgtgctag acatcagcaa atgcaaagaa gggagacctg
     1921 tgtacatttc acttcctcat tttctgtatg caagtcctga tgtttcagaa cctattgatg
     1981 gattaaaccc aaatgaagaa gaacatagga catacttgga tattgaacct ataactggat
     2041 tcactttaca atttgcaaaa cggctgcagg tcaacctatt ggtcaagcca tcagaaaaaa
     2101 ttcaagtatt aaagaatctg aagaggaact atattgtgcc tattctttgg cttaatgaga
     2161 ctgggaccat tggtgatgag aaggcaaaca tgttcagaag tcaagtaact ggaaaaataa
     2221 acctccttgg cctgatagaa atgatcttac tcagtgttgg tgtggtgatg tttgttgctt
     2281 ttatgatttc atattgtgca tgcagatcga aaacaataaa ataagaattc tgcagtcgac
     2341 ggtaccgcgg gcccgggatc caccggatct aagataactg atcataatca gccataccac
     2401 attt
//
