LOCUS       sequence-85266-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CEBPA237-C-4
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..726
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     432..451
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      301 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
      361 gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc cgaggagcgg
      481 atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa gctgaaggac
      541 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtccg gacctgcaat gggtgctgcg ggcctgccgg gccccggggg ctcgctcaag
      781 ggcttggctg gtccgcaccc cgacctccgc accggcggcg gcggcggcgg cggggccggc
      841 gcgggcaagg ccaagaagtc ggtggataag aacagcaacg agtaccgggt acggcgggaa
      901 cgcaacaaca tcgcggtgcg caagagccga gataaagcca aacagcgcaa cgtggagacg
      961 cagcagaagg tgttggagtt gaccagtgac aatgaccgcc tgcgcaagcg ggtggaacag
     1021 ctgagccgtg aactggacac gctgcggggt atcttccgcc agctgcctga gagctccttg
     1081 gtcaaggcca tgggcaactg cgcgtgaggc gcgcggatcc accggatcta gataactgat
     1141 cataatcagc c
//
