LOCUS       sequence-85268-e        1314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1314)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Centrin2-N-10
FEATURES             Location/Qualifiers
     source          1..1314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      559..568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             565..1275
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     978..997
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag ccgccaccat ggcctccaac tttaagaagg caaacatggc atcaagttct
       61 cagcgaaaaa gaatgagccc taagcctgag cttactgaag agcaaaagca ggagatccgg
      121 gaagcttttg atcttttcga tgcggatgga actggcacca tagatgttaa agaactgaag
      181 gtggcaatga gggccctggg ctttgaaccc aagaaagaag aaattaagaa aatgataagt
      241 gaaattgata aggaagggac aggaaaaatg aactttggtg actttttaac tgtgatgacc
      301 cagaaaatgt ctgagaaaga tactaaagaa gaaatcctga aagctttcaa gctctttgat
      361 gatgatgaaa ctgggaagat ttcgttcaaa aatctgaaac gcgtggccaa ggagttgggt
      421 gagaacctga ctgatgagga gctgcaggaa atgattgatg aagctgatcg agatggagat
      481 ggagaggtca gtgagcaaga gttcctgcgc atcatgaaaa agaccagcct ctattcggga
      541 agcacggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
      601 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      661 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
      721 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      781 ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
      841 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt
      901 aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct gcgcggcacc
      961 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccgag
     1021 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag gctgaagctg
     1081 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     1141 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
     1201 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1261 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca catt
//
