LOCUS       sequence-85269-e        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
      781 agtcgacatg gctgatgact ttggcttctt ctcgtcgtcg gagagtggtg ccccggaggc
      841 ggcggaggag gacccggcgg ccgccttcct ggcccagcag gagagcgaga ttgcaggcat
      901 agagaacgac gagggcttcg gggcacctgc cggcagccat gcggcccccg cacagccggg
      961 ccccacgagt ggggctggtt ctgaggacat ggggaccaca gtcaatggag atgtgtttca
     1021 ggaggccaac ggtcctgctg atggctacgc agccattgcc caggctgaca ggctgaccca
     1081 ggagcctgag agcatccgca agtggcgaga ggagcagagg aaacggctgc aagagctgga
     1141 tgctgcatct aaggtcacgg aacaggaatg gcgggagaag gccaagaagg acctggagga
     1201 gtggaaccag cgccagagtg aacaagtaga gaagaacaag atcaacaacc gggcatccga
     1261 ggaggctttc gtgaaggaat ccaaggagga gaccccaggc acagagtggg agaaggtggc
     1321 ccagctatgt gacttcaacc ccaagagcag caagcagtgc aaagatgtgt cccgcctgcg
     1381 ctcggtgctc atgtccctga agcagacgcc actgtcccgc tagggatcca ccggatctag
     1441 ataactgatc ataatcagcc ataccacatt
//
