LOCUS       sequence-85271-e        2154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Fong JT, Kells RM, Gumpert AM, Marzillier JY, Davidson MW, Falk MM
  TITLE     Internalized gap junctions are degraded by autophagy.
  JOURNAL   Autophagy. 2012 May 1;8(5):794-811. doi: 10.4161/auto.19390. Epub 
            2012 May 1.
  PUBMED    22635056
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4161/auto.19390"; journalName: "Autophagy"; date: "2012-05-1- 
            1"; volume: "8"; issue: "5"; pages: "794-811"
COMMENT     Sequence Label: mApple-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1399..1408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1405..2115
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1818..1837
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgg cagcactttt
       61 ctttcattgg ggggaaggcg tgaggaaagt accaaacagc agcagacttt taaactttaa
      121 acagacaggt ctgagagcct gaactctcat ttttcctttg acttcagcct ccaaggagtt
      181 ccaccaactt tggcgcgccg gcttcacttt cattaagtga aagagaggtg cccagacatg
      241 ggtgactgga gtgccttggg gaagcttctg gacaaggtcc aagcctactc caccgctgga
      301 gggaaggtgt ggctgtcagt gctcttcata ttcagaatcc tgctcctggg gacagctgtt
      361 gagtcagctt ggggtgatga acagtctgcc tttcgctgta acactcaaca acctggctgc
      421 gaaaacgtct gctatgacaa gtccttcccc atctctcacg tgcgcttctg ggtccttcag
      481 atcatattcg tgtctgtgcc cacactcctg tacttggccc atgtgttcta tgtgatgagg
      541 aaggaagaga agctaaacaa gaaagaagag gagctcaaag tggcccagac tgacggggtc
      601 aacgtggaga tgcacctgaa gcagattgaa atcaagaagt tcaagtacgg gattgaagag
      661 cacggcaagg tgaaaatgag gggcggcttg ctgagaacct acatcatcag catcctcttc
      721 aagtctgtct tcgaggtggc cttcctgctc atccagtggt acatctatgg gttcagcttg
      781 agcgcggtct acacctgcaa gagagatccc tgcccccacc aggtagactg cttcctctca
      841 cgtcccacgg agaaaaccat cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc
      901 gctttgaaca tcattgagct cttctacgtc ttcttcaaag gcgttaagga tcgcgtgaag
      961 ggaagaagcg atccttacca cgccaccact ggcccactga gcccatcaaa agactgcgga
     1021 tctccaaaat acgcctactt caatggctgc tcctcaccaa cggctccact ctcgcctatg
     1081 tctcctcctg ggtacaagct ggttactggt gacagaaaca attcctcgtg ccgcaattac
     1141 aacaagcaag ctagcgagca aaactgggcg aactacagcg cagagcaaaa tcgcatgggg
     1201 caggccggaa gcaccatctc caactcgcac gcccagccgt tcgatttccc cgacgacaac
     1261 cagaatgcca aaaaagttgc tgctggacat gaactccagc cattagccat cgtggaccaa
     1321 cgaccttcca gcagagccag cagccgcgcc agcagcaggc ctcggcctga tgacctggag
     1381 attgcggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
     1441 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1501 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
     1561 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1621 ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
     1681 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt
     1741 aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct gcgcggcacc
     1801 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccgag
     1861 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag gctgaagctg
     1921 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     1981 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
     2041 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     2101 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca catt
//
