LOCUS       sequence-85273-e        1545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1545)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..728
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     434..453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttgcgctacc ggtcgccacc atggtgagca agggcgagga gaataacatg gccatcatca
       61 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      121 tcgagggcga gggcgagggc cgcccctacg aggcctttca gaccgctaag ctgaaggtga
      181 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      241 ccaaggtcta cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg
      301 agggcttcag gtgggagcgc gtgatgaact tcgaggacgg cggcattatt cacgttaacc
      361 aggactcctc cctgcaggac ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact
      421 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccgaggagc
      481 ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa gaagaggctg aagctgaagg
      541 acggcggcca ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      601 tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt gtcccacaac gaggactaca
      661 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      721 tgtacaagtc cggactcaga tctggcagca gtggaggtga atatcatcct gatttagaaa
      781 atttggatga agatggatat actcaattac acttcgactc tcaaagcaat accaggatag
      841 ctgttgtttc agagaaagga tcgtgtgctg catctcctcc ttggcgcctc attgctgtaa
      901 ttttgggaat cctatgcttg gtaatactgg tgatagctgt ggtcctgggt accatggcta
      961 tttggagatc caattcagga agcaacacat tggagaatgg ctactttcta tcaagaaata
     1021 aagagaacca cagtcaaccc acacaatcat ctttagaaga cagtgtgact cctaccaaag
     1081 ctgtcaaaac cacaggggtt ctttccagcc cttgtcctcc taattggatt atatatgaga
     1141 agagctgtta tctattcagc atgtcactaa attcctggga tggaagtaaa agacaatgct
     1201 ggcaactggg ctctaatctc ctaaagatag acagctcaaa tgaattggga tttatagtaa
     1261 aacaagtgtc ttcccaacct gataattcat tttggatagg cctttctcgg ccccagactg
     1321 aagtaccatg gctctgggag gatggatcaa cattctcttc taacttattt cagatcagaa
     1381 ccacagctac ccaagaaaac ccatctccaa attgtgtatg gattcacgtg tcagtcattt
     1441 atgaccaact gtgtagtgtg ccctcatata gtatttgtga gaagaagttt tcaatgtaag
     1501 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gaatt
//
