LOCUS       sequence-85275-e        3510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3510)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmocollin1-C-18
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc
      301 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac
      361 gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag
      541 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct agtagcggag gtagcgcatc cggcggaagc
      781 ggaggaagcg ctctggcctc tgctgcccca gggagcatct tctgtaagca gctccttttc
      841 tctctcctgg ttttaacatt actttgcgat gcttgtcaga aagtttatct tcgagttcct
      901 tctcatcttc aggctgaaac acttgtaggc aaagtgaatc tggaggagtg tctcaagtcg
      961 gccagcctaa tccggtccag tgaccctgcc ttcaggattc tagaagatgg ctcaatttac
     1021 acaacacatg acctcatttt gtcttctgaa aggaaaagtt tttccatttt cctttcagat
     1081 ggtcagagac gggaacaaca agagataaaa gttgtactgt cagcaagaga aaacaagtct
     1141 cctaagaaga gacataccaa agacacagcc ctcaagcgca gcaagagacg atgggctcct
     1201 attccagctt cattgatgga gaactcgttg ggtccatttc cacaacacgt tcagcagatc
     1261 caatctgatg ctgcacagaa ttacaccatc ttttattcca taagtgggcc aggcgtggac
     1321 aaagaaccct tcaatttgtt ttacatagag aaagacactg gggatatctt ttgtacaagg
     1381 agcattgacc gtgagaaata cgaacagttt gcgttatatg gctatgcaac aactgcagat
     1441 ggctatgcac cagaatatcc actccctttg atcatcaaaa ttgaagatga taatgataac
     1501 gccccatatt ttgaacacag agtgactatc tttactgtgc ctgaaaattg ccgatccggc
     1561 acttcagtgg gaaaagtgac cgccacagac cttgacgaac ctgacactct ccatactcgt
     1621 ctgaaatata aaatcttaca acaaatccca gatcatccaa agcatttctc catacaccca
     1681 gataccggag tcatcaccac aactacacct tttctggata gagaaaaatg tgatacttac
     1741 cagttaataa tggaagtgcg agacatgggt ggtcagcctt tcggtttatt taatacagga
     1801 acaattacta tttcacttga ggatgaaaat gacaatccac catctttcac agaaacttct
     1861 tatgttacag aagtagaaga aaacagaatt gacgtggaga ttttgcgaat gaaggtacag
     1921 gatcaggatt tgccaaacac tcctcactca aaggctgtat acaaaatctt acaaggaaat
     1981 gaaaatggaa acttcataat tagcacagat ccaaatacaa atgaaggagt gctgtgtgtt
     2041 gtcaagccat tgaactatga agtcaatcgc caagttattt tgcaagttgg tgtcattaac
     2101 gaggcacaat tctctaaagc agcgagctca caaactccta caatgtgcac tacaactgtc
     2161 accgttaaaa ttatagacag tgatgagggc cctgaatgcc accctccagt gaaagttatt
     2221 cagagtcaag atggcttccc agctggccaa gaactccttg gatacaaagc actggacccg
     2281 gaaatatcca gtggtgaagg cttaaggtat cagaagttag gggatgaaga taactggttt
     2341 gaaattaatc aacacactgg cgacttgaga actctaaaag tactagatag agaatccaaa
     2401 tttgtaaaaa acaaccaata caatatttca gttgttgcag tggatgcagt tggccgatct
     2461 tgcactggaa cattagtagt tcatttggat gattacaacg atcacgcacc tcaaattgac
     2521 aaagaagtga ccatttgtca gaataatgag gattttgctg ttctgaaacc tgtagatcca
     2581 gatggacctg aaaatggacc accttttcaa ttctttctgg ataattctgc cagtaaaaac
     2641 tggaacatag aagaaaagga tggtaaaact gccattcttc gtcaacggca aaatcttgat
     2701 tataactatt attctgtgcc tattcaaata aaagacaggc atggtttagt tgcaacacat
     2761 atgttaacag tgagagtatg tgactgttca actccatctg agtgtagaat gaaggataaa
     2821 agtacaagag acgttagacc aaatgtaata cttggaagat gggctattct tgctatggtg
     2881 ttgggttctg tattgttatt atgtattctg tttacatgtt tctgtgtcac tgctaagaga
     2941 acagtcaaga aatgttttcc agaagacata gcccagcaaa atttaattgt atcaaatact
     3001 gaaggacctg gagaagaagt aacggaagca aatattagac tccccatgca gacatccaac
     3061 atttgtgaca caagcatgtc tgttggtact gttggtggcc agggaatcaa aacacagcaa
     3121 agttttgaga tggtcaaagg aggctacact ttggattcca acaaaggagg tggacatcag
     3181 accttggagt ccgtcaaggg agtggggcag ggagatactg gcagatatgc gtacacggac
     3241 tggcagagtt tcacccaacc tcggcttggc gaaaaggtgt atttgtgtgg acaagatgag
     3301 gagcataaac attgtgaaga ctacgtttgt tcgtataact atgaaggcaa aggttctctg
     3361 gccggctcag taggttgctg cagcgatcgg caggaagaag agggactgga gtttctagat
     3421 cacctggaac ccaaatttag gacattagca aagacatgca tcaagaaata aggatccacc
     3481 ggatctagat aactgatcat aatcagccat
//
